Background: Photoacoustic Microscopy (PAM) integrates optical and acoustic imaging, offering enhanced penetration depth for detecting optical-absorbing components in tissues. Nonetheless, challenges arise in scanning large areas with high spatial resolution. With speed limitations imposed by laser pulse repetition rates, the potential role of computational methods is highlighted in accelerating PAM imaging. Purpose: We are proposing a novel and highly adaptable DiffPam algorithm that utilizes diffusion models for speeding up the photoacoustic imaging process. Method: We leveraged a diffusion model trained exclusively on natural images, comparing its performance with an in-domain trained U-Net model using a dataset focused on PAM images of mice brain microvasculature. Results: Our findings indicate that DiffPam achieves comparable performance to a dedicated U-Net model, without the need for a large dataset or training a deep learning model. The study also introduces the efficacy of shortened diffusion processes for reducing computing time without compromising accuracy. Conclusion: This study underscores the significance of DiffPam as a practical algorithm for reconstructing undersampled PAM images, particularly for researchers with limited AI expertise and computational resources.

Like all other imaging modalities, the resolution, speed, sample preservation, and signal-to-noise ratio (SNR) of point scanning systems are difficult to optimize simultaneously. In particular, point scanning systems are uniquely constrained by an inverse relationship between imaging speed and pixel resolution. Here we show these limitations can be mitigated via the use of deep learning-based super-sampling of undersampled images acquired on a point-scanning system, which we termed point-scanning super-resolution (PSSR) imaging. Oversampled, high SNR ground truth images acquired on scanning electron or Airyscan laser scanning confocal microscopes were'crappified' to generate semi-synthetic training data for PSSR models that were then used to restore real-world undersampled images. Remarkably, our EM PSSR model could restore undersampled images acquired with different optics, detectors, samples, or sample preparation methods in other labs.